ALOX5 Antikörper (pSer523)

Produktnummer ABIN372586

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ALOX5 in WB. Er zeigt eine Reaktivität gegenüber Human, Ratte und Primate.

Kurzübersicht für ALOX5 Antikörper (pSer523) (ABIN372586)

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Reaktivität: Human, Ratte, Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ALOX5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-ALOX5 Antikörper

Bindungsspezifität: pSer523

Spezifität: Recognizes the ~80k 5-LO phosphorylated at Ser523 in Western blots.

Kreuzreaktivität (Details): Species reactivity (expected):non-Human Primates.
Species reactivity (tested):Human and Rat.

Aufreinigung: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser523 of human 5-Lipoxygenase (5-LO).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Andere Bezeichnung: ALOX5 / LOG5

Hintergrund: The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.Synonyms: 5-LO, 5-lipoxygenase, Arachidonate 5-lipoxygenase

Gen-ID: 240

NCBI Accession: NP_000689

UniProt: P09917