CA2 Antikörper (HRP)

Produktnummer ABIN372476

Der Kaninchen Polyklonal anti-CA2 Antikörper wird verwendet zum Nachweis von CA2 in Proben von Human und Kaninchen. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für CA2 Antikörper (HRP) (ABIN372476)

Target: CA2 (Carbonic Anhydrase II (CA2))

Reaktivität: Human, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CA2 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-CA2 Antikörper (HRP)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti Rabbit serum, as well as purified and partially purified Carbonic Anhydrase II (Human Erythrocytes).

Kreuzreaktivität (Details): Species reactivity (expected):Rabbit.
Species reactivity (tested):Human.

Aufreinigung: Ion Exchange Chromatography.

Immunogen: Carbonic Anhydrase II (Human Erythrocytes).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1000-1/6000. Western blot. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, pH 7.2 containing 0.01 % Gentamicin Sulfate as preservative and 10 mg/mL BSA as stabilizer, before the addition of Glycerol to 40 %

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Andere Bezeichnung: Carbonic Anhydrase 2

Hintergrund: Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29 kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20μM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.Synonyms: CA-II, CA2, CAC, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II

Gen-ID: 760

NCBI Accession: NP_000058

UniProt: P00918