Acetylcholinesterase Antikörper (N-Term)

Produktnummer ABIN372290

Der Kaninchen Polyklonal Anti-Acetylcholinesterase-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, Acetylcholinesterase in Proben von Human zu detektieren.

Kurzübersicht für Acetylcholinesterase Antikörper (N-Term) (ABIN372290)

Target: Acetylcholinesterase (AChE)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Acetylcholinesterase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Acetylcholinesterase Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody recognizes Acetylcholinesterase (ACHE).

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to N-terminal residues of Human Acetylcholinesterase (N- terminal extended acetylcholinesterase).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Paraffin Sections: 20 μg/mL. Western Blot: 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS containing 0.01 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Andere Bezeichnung: Acetylcholinesterase

Hintergrund: Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.Synonyms: AChE, Acetylcholine Esterase, Acetylcholine-Esterase

Molekulargewicht: 68 kDa.

Gen-ID: 43

NCBI Accession: NP_000656

UniProt: P22303

Pathways: Skeletal Muscle Fiber Development