AIRE Antikörper (Internal Region)

Produktnummer ABIN372242

Der Ziege Polyklonal anti-AIRE Antikörper wird verwendet zum Nachweis von AIRE in Proben von Human, Ratte und Hund. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für AIRE Antikörper (Internal Region) (ABIN372242)

Target: AIRE (Autoimmune Regulator (AIRE))

Reaktivität: Human, Ratte, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser AIRE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-AIRE Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-KDVDLSQPRK GRKP

Spezifität: This antibody recognize Apeced Protein (AIRE).

Kreuzreaktivität (Details): Species reactivity (expected):Dog (Canine) and Rat.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to the Internal region of Human AIRE.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/32000. Western Blot: 0.5-1 μg/mL. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris Saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Andere Bezeichnung: AIRE / APECED

Hintergrund: AIRE is a transcriptional regulator that is thought to be important in controlling tissue-specific expression of genes in the thymus. The precise function of the protein is not well defined. It contains zinc finger motifs and isoform 1 is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described. The longer AIRE1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE3 includes the same exon 1-8-9 sequences as found in AIRE2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).Synonyms: Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, Autoimmune regulator

Gen-ID: 326

NCBI Accession: NP_000374

UniProt: O43918

Pathways: Chromatin Binding