INA Antikörper

Produktnummer ABIN371820

Der Maus Monoklonal anti-INA Antikörper (Klon ID2) (ABIN371820) detektiert spezifisch INA in WB, IF, IHC (p) und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Säugetier.

Kurzübersicht für INA Antikörper (ABIN371820)

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Reaktivität: Säugetier

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser INA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: ID2

Produktdetails anti-INA Antikörper

Spezifität: Specific for the ~66k alpha Internexin protein.

Kreuzreaktivität (Details): Species reactivity (tested):most Mammalian.

Produktmerkmale: Synonyms: Alpha-Inx, INA, NEF5, NF66, 66 kDa neurofilament protein, Neurofilament-66

Aufreinigung: Ig Fraction

Immunogen: Recombinant rat alpha-Internexin expressed in and purified from E. coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: 1/1,000. This antibody can be used on formalin-fixed cells in tissue culture, cryostat sections. The epitope recognized by the ID2 clone is in the C-terminal non-helical extension of theprotein and is unusually resistant to aldehyde fixation, so that this antibody is ideal forstudies of paraffin embedded formalin fixed histological sections. Western blotting: 1/2,000. Immunofluorescence: 1/250.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu INA

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Andere Bezeichnung: alpha-Internexin

Hintergrund: Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5).Synonyms: 66 kDa neurofilament protein, Alpha-Inx, INA, NEF5, NF66, Neurofilament-66

Gen-ID: 9118

UniProt: Q16352