MHC Class I H-2Db Antikörper

Produktnummer ABIN371165

Der Maus Monoklonal anti-MHC Class I H-2Db Antikörper wird verwendet zum Nachweis von MHC Class I H-2Db in Proben von Maus. Er wurde validiert für FACS.

Kurzübersicht für MHC Class I H-2Db Antikörper (ABIN371165)

Target: MHC Class I H-2Db

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS)

Klon: 28-14-8

Produktdetails

Spezifität: This antibody is specific to an epitope in the alpha 3 domain of H-2Db. It binds to the alpha 3 domain of H-2Db in the presence or absence of beta 2 microglobulin. (Ref.7,8) It cross reacts with the alpha 3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, (Ref.9-11) but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. (Ref.12). Customer feedback: Clone 28-14-8 does not cross-react with human cells.

Produktmerkmale: Synonyms: H-2D(B), H2-D1, H-2 class I histocompatibility antigen, D-B alpha chain

Endotoxin-Niveau: Low Endotoxin

Immunogen: C3H. SW mouse splenocytes. (Ref. 1,2)

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: < / = 1 μg/10^6 cells. Immunohistochemistry on Frozen Tissue Sections. Complement-mediated cytotoxicity.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS without preservatives.

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Antigendetails

Target: MHC Class I H-2Db

Andere Bezeichnung: MHC Class I H-2 Db

Hintergrund: The 'classical' MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-Microglobulin. (Ref.3,4) These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (Ref.5)Synonyms: D-B alpha chain, H-2 class I histocompatibility antigen, H-2D(B), H2-D1

Gen-ID: 14964

UniProt: P01899