MBP Antikörper

Produktnummer ABIN364503

Der Kaninchen Polyklonal anti-MBP Antikörper (ABIN364503) detektiert spezifisch MBP in WB, IHC und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für MBP Antikörper (ABIN364503)

Target: MBP (Myelin Basic Protein (MBP))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MBP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-MBP Antikörper

Spezifität: Suitable for labeling of oligodendrocytes and myelin of white matter in brain and spinal cord (gray matter stains less abundantly). Also stains peripheral nerves.

Aufreinigung: Ammonium sulfate precipitation

Immunogen: Human myelin basic protein from brain.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (1:100), WB (1:1500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100. Western Blot: 1:1500 in TTBS, overnight at 4uC.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.05M Tris HCL,  pH 7.6, 15 mM sodium azide before the addition of glycerol to 40 %

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu MBP

Target: MBP (Myelin Basic Protein (MBP))

Andere Bezeichnung: Myelin Basic Protein / MBP

Hintergrund: Name/Gene ID: MBP

Synonyms: MBP, Myelin A1 protein, Myelin basic protein

Gen-ID: 4155

UniProt: P02686