Catalase Antikörper (AA 498-511)

Produktnummer ABIN364408

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch Catalase in WB, IHC, ELISA und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Hund, Affe, Pferd, Fledermaus und Hamster.

Kurzübersicht für Catalase Antikörper (AA 498-511) (ABIN364408)

Target: Catalase (CAT)

Reaktivität: Human, Maus, Hund, Affe, Pferd, Fledermaus, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Catalase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Catalase Antikörper

Bindungsspezifität: AA 498-511

Marke: IHC-plus™

Spezifität: Human CAT / Catalase.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Panda, Bat, Dog, Horse (100%) Elephant, Pig, Guinea pig (93%) Sheep, Goat, Bovine (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-DKYNAEKPKNAIHT from an internal region of human CAT / Catalase (NP_001743.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Panda, Bat, Dog, Horse (100%), Elephant, Pig, Guinea pig (93%), Sheep, Goat, Bovine (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Catalase

Target: Catalase (CAT)

Andere Bezeichnung: CAT / Catalase

Hintergrund: Name/Gene ID: CAT

Synonyms: CAT, Catalase

Gen-ID: 847

NCBI Accession: NP_001743

UniProt: P04040

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism