Claudin 7 Antikörper (C-Term)

Produktnummer ABIN364312

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Claudin 7 in WB, ELISA, IHC, IHC (p) und IP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Claudin 7 Antikörper (C-Term) (ABIN364312)

Target: Claudin 7 (CLDN7)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Claudin 7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Produktdetails anti-Claudin 7 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Specific for the claudin-7 protein and does not cross-react with Claudin-3 or -4. On Western blots, it identifies a band at ~22kD. Reactivity has been confirmed with T47D cells by Western blotting and with formalin-fixed, paraffin-embedded human breast cancer tissues by immunohistochemistry.

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide derived from the C-terminal region of the human Claudin-7 protein.

Type of Immunogen: Synthetic peptide

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (10 μg/mL), IP, WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: Western Blot: T47D cells.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Claudin 7

Target: Claudin 7 (CLDN7)

Andere Bezeichnung: CLDN7 / Claudin 7

Hintergrund: Name/Gene ID: CLDN7
Family: Claudin

Synonyms: CLDN7, Claudin-7, Claudin-1, CLDN-7, Hs.84359, CEPTRL2, Claudin 7, CPETRL2

Gen-ID: 1366

UniProt: O95471

Pathways: Hepatitis C