TJP3 Antikörper (C-Term)

Produktnummer ABIN364296

Dieses Anti-TJP3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TJP3 in WB, IP, ELISA, IHC, IF und IHC (p). Geeignet für Human und Hund.

Kurzübersicht für TJP3 Antikörper (C-Term) (ABIN364296)

Target: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Reaktivität: Human, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TJP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-TJP3 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Reacts with ZO-3 protein with a molecular weight of ~130kD. Cross-reactivity with other Zonula Occluden family members has not been observed. Reactivity is confirmed with human T84 and MDCK cell lysates. Species cross-reactivity: human and canine.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminus of the human ZO-3 protein.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IF, IHC, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu TJP3

Target: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Andere Bezeichnung: TJP3 / ZO3

Hintergrund: Name/Gene ID: TJP3
Family: Occludin

Synonyms: TJP3, Tight junction protein 3, ZO-3, Zona occludens protein 3, Zonula occludens protein 3, Tight junction protein ZO-3, ZO3

Gen-ID: 27134

UniProt: O95049