HSPB6 Antikörper

Produktnummer ABIN364293

Dieses Anti-HSPB6-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von HSPB6 in WB, IHC, IP und IHC (p). Geeignet für Human, Ratte, Maus, Rind (Kuh) und Kaninchen.

Kurzübersicht für HSPB6 Antikörper (ABIN364293)

Target: HSPB6 (Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSPB6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HSPB6 Antikörper

Marke: IHC-plus™

Spezifität: Recognizes HSP20, Mr ~20kD. Species cross-reactivity: Human, mouse, rabbit, rat and bovine.

Aufreinigung: Immunoaffinity purified

Immunogen: Native HSP20 purified from human skeletal muscle.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot Analysis: 1 μg/mL detected HSP20 in RIPA lysates from rabbit skeletal muscle and mouse heart cytosol. Immunoprecipitation: Reported to immunoprecipitate HSP20 from rat heart extract and lysates of bovine carotid artery smooth muscle.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M PBS,  pH 7.0, 0.1 % sodium azide, 30 mg/mL BSA, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu HSPB6

Target: HSPB6 (Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6))

Andere Bezeichnung: HSPB6 / HSP20

Hintergrund: Name/Gene ID: HSPB6

Synonyms: HSPB6, Heat shock protein beta-6, Hsp20

Gen-ID: 126393

UniProt: O14558