SIRT6 Antikörper (C-Term)

Produktnummer ABIN363528

Dieses Anti-SIRT6-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SIRT6 in WB, ICC und IF. Geeignet für Human.

Kurzübersicht für SIRT6 Antikörper (C-Term) (ABIN363528)

Target: SIRT6 (Sirtuin 6 (SIRT6))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIRT6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Produktdetails anti-SIRT6 Antikörper

Bindungsspezifität: AA 300-355, C-Term

Spezifität: Reacts with human SIRT6 protein

Kreuzreaktivität (Details): 72 % sequence identity with rat.

Aufreinigung: Antigen affinity purified

Immunogen: Synthetic peptide made to a C-terminal region of the human SIRT6 protein (within residues 300-355)

Anwendungsinformationen

Applikationshinweise:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
- WB : 2 μg/mL - ICC/IF: 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.05 mg/mL

Buffer: Tris-citrate, phosphate, ( pH 7-8) buffer, Sodium azide 0.1 %

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C. Do not freeze.

Details zu SIRT6

Target: SIRT6 (Sirtuin 6 (SIRT6))

Hintergrund: SIRT6 (SIR2-like protein 6) is a member of the yeast silent information regulator 2 (SIR2) family and possesses both strong ADP-ribosyltransferase activity as well as protein deacetylase activity which is dependent on NAD(+). SIRT6 exerts deacetylase activity towards histone H3K9Ac and H3K56Ac, and alters histone H3 acetylation in telomeric chromatin during cell cycle's S-phase. It deacetylates histone H3K9Ac at NFkB target promoters and downregulates a subset of NFkB target genes. Upon DNA damage, SIRT6 promotes DNA end resection via deacetylation of RBBP8. SIRT6 also serves as a corepressor of the transcription factor HIF1 alpha for regulating the expression of multiple glycolytic genes to control glucose homeostasis, and is required for normal IGF1 serum levels. SIRT6 play critical roles in maintaining telomere integrity, fine-tuning aging-associated gene expression programs, preventing genomic instability, and maintaining metabolic homeostasis, thus impacting on several pathways relevant for cancer, metabolism, aging, cellular senescence and apoptosis.