OCT4 Antikörper (AA 100-200)

Produktnummer ABIN363517

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch OCT4 in WB, IF, ICC und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Affe.

Kurzübersicht für OCT4 Antikörper (AA 100-200) (ABIN363517)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reaktivität: Human, Maus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OCT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-OCT4 Antikörper

Bindungsspezifität: AA 100-200

Spezifität: Reacts with Oct-4 protein from human, mouse, rat and primate

Kreuzreaktivität: Rind (Kuh), Schwein

Kreuzreaktivität (Details): Predicted to cross react with bovine (93 % ), feline (93 %), porcine (93 %).

Aufreinigung: Purified

Immunogen: Synthetic peptide made to an internal portion of the human OCT4 protein sequence (between residues 100-200)

Anwendungsinformationen

Applikationshinweise:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
- WB : 2 μg/mL - FC: 1:50-1:200 -ICC/IF, 1:50-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.85 mg/mL

Buffer: Tris-glycine, NaCl 150 mM buffer, Sodium azide 0.05 %

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Details zu OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Andere Bezeichnung: Octamer-Binding Protein 4 (Oct-4)

Hintergrund: Stem cells are crucial ingredients in the progressive restriction of cellular developmental capacity during mammalian embryogenesis. Until recently, only embryonic stem cells (ESC)had been shown to exhibit the potential to differentiate into cells of the all three germ layers. However, adult stem cells (ASC) can regain pluripotency (potential to differentiate into various lineages) if transferred from one stem-cell microenvironment into another.The transcription factor OCT4, expressed in ESCs and germ cells, is strongly implicated in the process of maintaining as well as regaining stem-cell pluripotency and functions as a key regulator of mammalian germline development.

UniProt: Q01860

Pathways: Stem Cell Maintenance