p300 Antikörper (AA 1572-2371)

Produktnummer ABIN363282

Dieses Anti-p300-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von p300 in WB, IP, ICC und IF. Geeignet für Human, Maus, Ratte, Affe und Nerz.

Kurzübersicht für p300 Antikörper (AA 1572-2371) (ABIN363282)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reaktivität: Human, Maus, Ratte, Affe, Nerz

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p300 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: RW128

Produktdetails anti-p300 Antikörper

Bindungsspezifität: AA 1572-2371

Spezifität: Reacts with human p300 protein (residues 1868-1921)

Kreuzreaktivität: Maus

Kreuzreaktivität (Details): Cross reacts with proteins from mouse, rat, monkey and mink.

Aufreinigung: Purified (protein A)

Immunogen: Fusion protein containing residues 1572-2371 of human p300

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ICC/IF1:10-1:2000, IP1:10-1:500, WB1:250-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.4, Sodium azide 0.1 %

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Details zu p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Andere Bezeichnung: p300

Hintergrund: Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.

Gen-ID: 2033

UniProt: Q09472

Pathways: p53 Signalweg, Notch Signalweg, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size