Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-IRS1 Antikörper wird verwendet zum Nachweis von IRS1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC und IF.
The antibody detects endogenous level of total IRS-1 protein.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Immunogen
Peptide sequence around AA 634-638 (P-M-S-P-K) derived from Human IRS-1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Immunofluorescence: 1:100-1:200
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Ekuni, Tomofuji, Irie, Kasuyama, Umakoshi, Azuma, Tamaki, Sanbe, Endo, Yamamoto, Nishida, Morita: "Effects of periodontitis on aortic insulin resistance in an obese rat model." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 90, Issue 3, pp. 348-59, (2010) (PubMed).
Target
IRS1
(Insulin Receptor Substrate 1 (IRS1))
Andere Bezeichnung
IRS-1
Hintergrund
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit