The antibody detects endogenous level of MARCKS only when phosphorylated at serine 158.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer158 (R-F-S (p) -F-K) derived from Human MARCKS. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
MARCKS
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
RB04646
unconjugated
Applikationshinweise
Western blotting: 1:500-1:1000 Immunofluorescence: 1:100-1:200
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target
MARCKS
(Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
Andere Bezeichnung
MARCKS
Hintergrund
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.