Der Kaninchen Polyklonal anti-MARCKS Antikörper (ABIN362380) detektiert spezifisch MARCKS in WB und IF.
Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.
The antibody detects endogenous level of MARCKS only when phosphorylated at serine 158.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer158 (R-F-S (p) -F-K) derived from Human MARCKS. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
MARCKS
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
RB04646
unconjugated
Applikationshinweise
Western blotting: 1:500-1:1000 Immunofluorescence: 1:100-1:200
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target
MARCKS
(Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
Andere Bezeichnung
MARCKS
Hintergrund
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.