The antibody detects endogenous level of HSF1 only when phosphorylated at serine 303.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer303 (P-P-S (p) -P-P) derived from Human HSF1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target
HSF1
(Heat Shock Factor Protein 1 (HSF1))
Andere Bezeichnung
HSF1
Hintergrund
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.