SAPK, JNK (pThr183) Antikörper

Produktnummer ABIN362308

Zitiert in 2+ Publikationen. Der Kaninchen Polyklonal anti-SAPK, JNK Antikörper (ABIN362308) detektiert spezifisch SAPK, JNK in IHC und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für SAPK, JNK (pThr183) Antikörper (ABIN362308)

Target: SAPK, JNK

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails

Bindungsspezifität: pThr183

Spezifität: The antibody detects endogenous level of SAPK/JNK only when phosphorylated at threonine 183.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr183 (M-M-T (p) -P-Y) derived from Human SAPK, JNK. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen

Jiao, Zhou, Yang, Zhao, Liu, Yuan, Wang: "MK-2206 induces cell cycle arrest and apoptosis in HepG2 cells and sensitizes TRAIL-mediated cell death." in: Molecular and cellular biochemistry, Vol. 382, Issue 1-2, pp. 217-24, (2013) (PubMed).

Thévenin, Zony, Bahnson, Colman: "Activation by phosphorylation and purification of human c-Jun N-terminal kinase (JNK) isoforms in milligram amounts." in: Protein expression and purification, Vol. 75, Issue 2, pp. 138-46, (2010) (PubMed).

Antigendetails

Target: SAPK, JNK

Andere Bezeichnung: SAPK/JNK

Hintergrund: Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.

Molekulargewicht: 54 kDa

NCBI Accession: NP_001128

UniProt: P53779