Histone H3.1 Antikörper (pSer10)

Produktnummer ABIN362204

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Histone H3.1 in WB, IF und IHC. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte. Es wurde in 4+ Publikationen zitiert.

Kurzübersicht für Histone H3.1 Antikörper (pSer10) (ABIN362204)

Target: Histone H3.1 (HIST1H3B)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H3.1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Produktdetails anti-Histone H3.1 Antikörper

Bindungsspezifität: pSer10

Spezifität: The antibody detects endogenous level of Histone H3.1 only when phosphorylated at serine 10.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer10 (R-K-S (p) -T-G) derived from Human Histone H3.1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-Histone H3.1 Antikörper (ABIN362204)

Jiang, Li, Yang, Hawke, Zheng, Xia, Aldape, Wei, Guo, Chen, Lu: "PKM2 regulates chromosome segregation and mitosis progression of tumor cells." in: Molecular cell, Vol. 53, Issue 1, pp. 75-87, (2014) (PubMed).

Liao, Dong, Wu, Yang, Wang, Guo, Chen, Xu, Wen: "Resolvin D1 attenuates inflammation in lipopolysaccharide-induced acute lung injury through a process involving the PPAR?/NF-?B pathway." in: Respiratory research, Vol. 13, pp. 110, (2013) (PubMed).

Sun, Chen, Wang, Li, Xue, Qu, Zhang, Mu, Lu: "The protective effect of alpha lipoic acid on Schwann cells exposed to constant or intermittent high glucose." in: Biochemical pharmacology, Vol. 84, Issue 7, pp. 961-73, (2012) (PubMed).

Yang, Xia, Hawke, Li, Liang, Xing, Aldape, Hunter, Alfred Yung, Lu: "PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis." in: Cell, Vol. 150, Issue 4, pp. 685-96, (2012) (PubMed).

Details zu Histone H3.1

Target: Histone H3.1 (HIST1H3B)

Andere Bezeichnung: Histone H3.1

Hintergrund: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Molekulargewicht: 17 kDa

NCBI Accession: NP_003521

UniProt: P68431