PAK1/2/3 Antikörper (pThr423)

Produktnummer ABIN362174

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PAK1/2/3 in IHC und IF. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für PAK1/2/3 Antikörper (pThr423) (ABIN362174)

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAK1/2/3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-PAK1/2/3 Antikörper

Bindungsspezifität: pThr423

Spezifität: The Antibody detects endogenous level of p90RSK only when phosphorylated at serine 352.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr423, 402, 421 (R-S-T (p) -M-V) derived from Human PAK1, PAK2, PAK3. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Details zu PAK1/2/3

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Andere Bezeichnung: PAK1/PAK2/PAK3

Hintergrund: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

NCBI Accession: NP_001121, NP_002568

UniProt: O75914