IRS1 Antikörper (pSer312)

Produktnummer ABIN362133

Dieses Anti-IRS1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von IRS1 in WB. Geeignet für Human, Maus und Ratte.

Kurzübersicht für IRS1 Antikörper (pSer312) (ABIN362133)

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IRS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-IRS1 Antikörper

Bindungsspezifität: pSer312

Spezifität: The antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 312.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer312 (A-T-S (p) -P-A) derived from Human IRS-1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Details zu IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Andere Bezeichnung: IRS-1

Hintergrund: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit

Molekulargewicht: 180 kDa

NCBI Accession: NP_005535

UniProt: P35568

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process