Cofilin Antikörper (pSer3)

Produktnummer ABIN362130

Es sind 4+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-Cofilin Antikörper wird verwendet zum Nachweis von Cofilin in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC und IF.

Kurzübersicht für Cofilin Antikörper (pSer3) (ABIN362130)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cofilin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Cofilin Antikörper

Bindungsspezifität: pSer3

Spezifität: The antibody detects endogenous level of cofilin only when phosphorylated at serine 3.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer3 (M-A-S (p) -G-V) derived from Human cofilin. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-Cofilin Antikörper (ABIN362130)

Huet, Rajakylä, Viita, Skarp, Crivaro, Dopie, Vartiainen: "Actin-regulated feedback loop based on Phactr4, PP1 and cofilin maintains the actin monomer pool." in: Journal of cell science, Vol. 126, Issue Pt 2, pp. 497-507, (2013) (PubMed).

Zhang, Luo, Wan, Wu, Laski, Chen: "Regulation of cofilin phosphorylation and asymmetry in collective cell migration during morphogenesis." in: Development (Cambridge, England), Vol. 138, Issue 3, pp. 455-64, (2011) (PubMed).

Guo, Lv, Tian, Hu, Wang, Sui, Jiang, Ruan, Nan: "Downregulation of p57 accelerates the growth and invasion of hepatocellular carcinoma." in: Carcinogenesis, Vol. 32, Issue 12, pp. 1897-904, (2011) (PubMed).

Scott: "H. R. Morgan, Astronomer." in: Science (New York, N.Y.), Vol. 126, Issue 3272, pp. 497, (2010) (PubMed).

Details zu Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Andere Bezeichnung: cofilin

Hintergrund: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Molekulargewicht: 19 kDa

NCBI Accession: NP_005498

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events