tau Antikörper (pThr205)

Produktnummer ABIN362074

Produktdetails anti-tau Antikörper

Target: tau (tau Protein)

Bindungsspezifität: pThr205

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser tau Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Spezifität: The antibody detects endogenous level of Tau only when phosphorylated at threonine 205.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr205 (P-G-T (p) -P-G) derived from Human Tau. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-tau Antikörper (ABIN362074)

Sun, Wei, Xiong, Wang, Xie, Wang, Yang, Wang, Lu, Liu, Wang: "Synaptic released zinc promotes tau hyperphosphorylation by inhibition of protein phosphatase 2A (PP2A)." in: The Journal of biological chemistry, Vol. 287, Issue 14, pp. 11174-82, (2012) (PubMed).

Chen, Xiong, Kong, Qu, Wang, Chen, Wang, Zhu: "Neuroglobin attenuates Alzheimer-like tau hyperphosphorylation by activating Akt signaling." in: Journal of neurochemistry, Vol. 120, Issue 1, pp. 157-64, (2011) (PubMed).

Tan, Cao, Wang, Lv, Wu, Ma: "Tau hyperphosphorylation is associated with memory impairment after exposure to 1.5% isoflurane without temperature maintenance in rats." in: European journal of anaesthesiology, Vol. 27, Issue 9, pp. 835-41, (2010) (PubMed).

Tan, Cao, Wang, Lv, Wu, Ma: "Protective effects of lithium treatment for spatial memory deficits induced by tau hyperphosphorylation in splenectomized rats." in: Clinical and experimental pharmacology & physiology, Vol. 37, Issue 10, pp. 1010-5, (2010) (PubMed).

Details zu tau

Target: tau (tau Protein)

Andere Bezeichnung: Tau (tau Produkte)

Synonyme: DDPAC antikoerper, FTDP-17 antikoerper, MAPTL antikoerper, MSTD antikoerper, MTBT1 antikoerper, MTBT2 antikoerper, PPND antikoerper, TAU antikoerper, tau antikoerper, PHF-tau antikoerper, microtubule associated protein tau antikoerper, MAPT antikoerper

Hintergrund: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Molekulargewicht: 78 kDa

UniProt: P10636