The antibody detects endogenous level of PKD/PKCm only when phosphorylated at serine 910.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer910 (R-V-S (p) -I-L) derived from Human PKD, PKCm. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
PRKD1
Reaktivität: Human
WB, ELISA, IHC
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western blotting: 1:500-1:1000 Immunofluorescence: 1:100-1:200
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target
PKC mu (PRKD1)
(Protein Kinase D1 (PRKD1))
Andere Bezeichnung
PKD/PKCmu
Hintergrund
Converts transient diacylglycerol. (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.