Estrogen Receptor alpha Antikörper (pSer167)

Produktnummer ABIN362012

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Estrogen Receptor alpha in WB, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human und Maus.Es wurde in 1 Publikation zitiert.

Kurzübersicht für Estrogen Receptor alpha Antikörper (pSer167) (ABIN362012)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Estrogen Receptor alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Estrogen Receptor alpha Antikörper

Bindungsspezifität: pSer167

Spezifität: The antibody detects endogenous level of Estrogen Receptor-a only when phosphorylated at serine16.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer167 (L-A-S (p) -T-N) derived from Human Estrogen Receptor-a. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-Estrogen Receptor alpha Antikörper (ABIN362012)

Motomura, Ishitobi, Komoike, Koyama, Nagase, Inaji, Noguchi et al.: "Expression of estrogen receptor beta and phosphorylation of estrogen receptor alpha serine 167 correlate with progression-free survival in patients with metastatic breast cancer treated with ..." in: Oncology, Vol. 79, Issue 1-2, pp. 55-61, (2011) (PubMed).

Details zu Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Andere Bezeichnung: Estrogen Receptor-alpha

Hintergrund: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

Molekulargewicht: 66 kDa

NCBI Accession: NP_000116

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly