Lysine (lys) (acetylated) Antikörper

Produktnummer ABIN361803

Der Maus Monoklonal Anti--Antikörper wurde für ICC, IF, IP, ELISA, WB und IHC validiert. Er ist geeignet, in Proben von zu detektieren.

Kurzübersicht für Lysine (lys) (acetylated) Antikörper (ABIN361803)

Target: Lysine (lys)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Klon: 7F8

Produktdetails

Bindungsspezifität: acetylated

Spezifität: Detects proteins containing acetylated lysine residues in ELISA and western blots. Does not detect non-acetylated lysine residues.

Aufreinigung: Protein G Purified

Immunogen: Acetylated KLH

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg of ABIN361802 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 μg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Antigendetails

Target: Lysine (lys)

Andere Bezeichnung: Lysine

Substanzklasse: Amino Acid

Hintergrund: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε development (5).