HSF1 Antikörper (AA 378-395)

Produktnummer ABIN361704

Der Ratte Monoklonal Anti-HSF1-Antikörper wurde für WB, ELISA, IHC, IP, IF, GS und ICC validiert. Er ist geeignet, HSF1 in Proben von Maus zu detektieren.

Kurzübersicht für HSF1 Antikörper (AA 378-395) (ABIN361704)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser HSF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Gel Shift (GS), Immunocytochemistry (ICC)

Klon: 10H8

Produktdetails anti-HSF1 Antikörper

Bindungsspezifität: AA 378-395

Spezifität: Detects ~85 kDa (unstressed cell lysates), and~95 kDa (heat shocked cell lysates).

Kreuzreaktivität: Rind (Kuh), Meerschweinchen, Hamster, Human, Affe, Maus, Kaninchen, Ratte

Aufreinigung: Protein G Purified

Immunogen: Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:200)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN361703 was sufficient for detection of HSF1 in 20 μg of heat shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Details zu HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Andere Bezeichnung: HSF1

Hintergrund: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

Gen-ID: 15499

NCBI Accession: NP_032322

UniProt: P38532