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DLG4 Antikörper

Dieses unabhängig validierte Maus Monoklonal-Antikörper erkennt spezifisch DLG4 in WB, IHC, ICC, IF, AA und mIHC. Er zeigt eine Reaktivität gegenüber Ratte.
Produktnummer ABIN361694

Kurzübersicht für DLG4 Antikörper (ABIN361694)

Target

Alle DLG4 Antikörper anzeigen
DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Reaktivität

  • 141
  • 125
  • 121
  • 29
  • 18
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Ratte

Wirt

  • 104
  • 73
  • 1
  • 1
  • 1
  • 1
Maus

Klonalität

  • 93
  • 86
  • 1
Monoklonal

Konjugat

  • 79
  • 9
  • 8
  • 8
  • 7
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Dieser DLG4 Antikörper ist unkonjugiert

Applikation

  • 138
  • 83
  • 50
  • 41
  • 40
  • 40
  • 28
  • 28
  • 20
  • 20
  • 19
  • 16
  • 9
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Array (AA), Multiplex Immunohistochemistry (mIHC)

Klon

6G6
  • Spezifität

    Detects ~100 kDa. An additional protein of >100 kDa is also detected. Additional cross-reactive bands are detected at ~75 kDa and 50 kDa in rat and mouse samples.

    Kreuzreaktivität

    Rind (Kuh), Human, Maus, Ratte

    Aufreinigung

    Protein G Purified

    Immunogen

    Recombinant rat PSD-95

    Isotyp

    IgG2a
  • Applikationshinweise

    • WB (1:250)
    • IHC (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.

    Kommentare

    1 μg/ml was sufficient for detection of PSD-95 on 20 μg rat brain tissue extract by ECL immunoblot analysis using Goat Anti-Mouse IgG: HRP as the secondary.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Validierung #104331 (Multiplex Immunohistochemistry)
    'Independent Validation' Siegel
    by
    Akoya Biosciences
    No.
    #104331
    Datum
    20.04.2021
    Antigen
    DLG4
    Chargennummer
    MP686150
    Validierte Anwendung
    Multiplex Immunohistochemistry
    Positivkontrolle

    Fresh frozen mouse olfactory bulb

    Negativkontrolle

    Unlabeled control (mouse fresh frozen)

    Bewertung

    Passed. The anti-DLG4 antibody ABIN361694 specifically labels areas that contain high levels of post-synaptic boutons; these are located throughout the murine olfactory bulb and are concentrated in the glomerular layer.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN361694
    Sekundärantikörper
    Full Protocol
    • Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
    • Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
    • Conjugation of the anti-DLG4 antibody ABIN361694 to an oligo barcode used to bind oligo-conjugated fluorophore ATTO 550.
    Anmerkungen
    • No signal was detected in unlabeled specimens.

    • Specific staining of DLG4 was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

    • Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for enogenous tissue autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    -20°C
  • Target

    DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

    Andere Bezeichnung

    PSD95

    Hintergrund

    Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90 kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex (1). These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins (2). Thus different PDZ domains of PSD-95 might be specialized for distinct functions (3, 4). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (5). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning (6). Mutations in PSD95 are associated with autism (7).

    Gen-ID

    29495

    NCBI Accession

    NP_062567

    UniProt

    P31016

    Pathways

    Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development, Asymmetric Protein Localization, Regulation of long-term Neuronal Synaptic Plasticity
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