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KDELR Antikörper (AA 192-212)

KDELR Reaktivität: Rind (Kuh) WB, IP, IHC, ICC, IF Wirt: Maus Monoclonal KR-10 unconjugated
Produktnummer ABIN361676
  • Target Alle KDELR Antikörper anzeigen
    KDELR (KDEL Receptor (KDELR))
    Bindungsspezifität
    • 20
    • 1
    AA 192-212
    Reaktivität
    • 23
    • 13
    • 13
    • 12
    • 12
    • 12
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 10
    • 2
    • 1
    Rind (Kuh)
    Wirt
    • 23
    • 1
    Maus
    Klonalität
    • 23
    • 1
    Monoklonal
    Konjugat
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Dieser KDELR Antikörper ist unkonjugiert
    Applikation
    • 23
    • 22
    • 22
    • 12
    • 10
    • 10
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Spezifität
    Detects ~25 kDa.
    Kreuzreaktivität
    Huhn, Rind (Kuh), Hund, Drosophila melanogaster, Hamster, Human, Affe, Maus, Schwein, Kaninchen, Ratte, Schaf, Xenopus laevis
    Aufreinigung
    Protein G Purified
    Immunogen
    A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH
    Klon
    KR-10
    Isotyp
    IgG1
  • Applikationshinweise
    • WB (1:1000)
    • ICC/IF (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    1 μg/ml was sufficient for detection of KDEL receptor in 20 μg monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    -20°C
  • Target
    KDELR (KDEL Receptor (KDELR))
    Andere Bezeichnung
    KDEL Receptor (KDELR Produkte)
    Synonyme
    BEST:CK00230 antikoerper, CG5183 antikoerper, CK00230 antikoerper, Dmel\\CG5183 antikoerper, ERD2 antikoerper, Erd2 antikoerper, dmErd2 antikoerper, l(2)k00311 antikoerper, KDEL receptor antikoerper, KdelR antikoerper
    Hintergrund
    The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity, and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).
    Gen-ID
    618184
    NCBI Accession
    NP_001069963
    UniProt
    P33946
    Pathways
    Maintenance of Protein Location
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