ALOX5 Antikörper (pSer523)

Produktnummer ABIN361506

Produktdetails anti-ALOX5 Antikörper

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Bindungsspezifität: pSer523

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ALOX5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: Specific for the ~80k 5-LO phosphorylated at Ser523 in Western blots.

Kreuzreaktivität: Human, Ratte (Rattus)

Homologie: non human primates

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser523 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Details zu ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Andere Bezeichnung: ALOX5 (ALOX5 Produkte)

Synonyme: 5-LO antikoerper, 5-LOX antikoerper, 5LPG antikoerper, LOG5 antikoerper, 5LO antikoerper, 5LX antikoerper, AI850497 antikoerper, F730011J02 antikoerper, LOX5A antikoerper, si:dkey-194n13.2 antikoerper, lox5 antikoerper, arachidonate 5-lipoxygenase antikoerper, arachidonate 5-lipoxygenase a antikoerper, Arachidonate 5-lipoxygenase antikoerper, ALOX5 antikoerper, Alox5 antikoerper, alox5a antikoerper, AFUA_4G02770 antikoerper, AOR_1_1362014 antikoerper, lox5 antikoerper

Hintergrund: The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis. Anti-Phospho Ser523 5-Lipoxygenase Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the 5-LO Ser523 antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Molekulargewicht: '80 kDa

Gen-ID: 240

UniProt: P09917