Tyrosine Hydroxylase Antikörper (pSer40)

Produktnummer ABIN361503

Dieser Anti-Tyrosine Hydroxylase Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von Tyrosine Hydroxylase in WB, IHC, ICC und ELISA. Geeignet für Ratte. Dieses Primary Antibody wurde in 12+ Publikationen zitiert.

Kurzübersicht für Tyrosine Hydroxylase Antikörper (pSer40) (ABIN361503)

Target: Tyrosine Hydroxylase (TH)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tyrosine Hydroxylase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA

Produktdetails anti-Tyrosine Hydroxylase Antikörper

Bindungsspezifität: pSer40

Spezifität: Specific for endogenous levels of the ~60 kDa tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over non-phospho TH.

Kreuzreaktivität: Säugetier, Diverse Spezies

Produktmerkmale: Our Tyrosine Hydroxylase (Ser40) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Tyrosine Hydroxylase (Ser40) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC, ICC.

Aufreinigung: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser40 of rat tyrosine hydroxylase, conjugated to keyhole limpet hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Dilution Range: ICC: 1:50-1:1000

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Haltbarkeit: 12 months

Referenzen für anti-Tyrosine Hydroxylase Antikörper (ABIN361503)

Salvatore, Calipari, Jones: "Regulation of Tyrosine Hydroxylase Expression and Phosphorylation in Dopamine Transporter-Deficient Mice." in: ACS chemical neuroscience, Vol. 7, Issue 7, pp. 941-51, (2017) (PubMed).

Salvatore, Terrebonne, Fields, Nodurft, Runfalo, Latimer, Ingram: "Initiation of calorie restriction in middle-aged male rats attenuates aging-related motoric decline and bradykinesia without increased striatal dopamine." in: Neurobiology of aging, Vol. 37, pp. 192-207, (2016) (PubMed).

Calipari, Ferris, Melchior, Bermejo, Salahpour, Roberts, Jones: "Methylphenidate and cocaine self-administration produce distinct dopamine terminal alterations." in: Addiction biology, Vol. 19, Issue 2, pp. 145-55, (2014) (PubMed).

Izumi, Ezumi, Takada-Takatori, Akaike, Kume: "Endogenous dopamine is involved in the herbicide paraquat-induced dopaminergic cell death." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 139, Issue 2, pp. 466-78, (2014) (PubMed).

Salvatore, Pruett: "Dichotomy of Tyrosine Hydroxylase and Dopamine Regulation between Somatodendritic and Terminal Field Areas of Nigrostriatal and Mesoaccumbens Pathways." in: PLoS ONE, Vol. 7, Issue 1, pp. e29867, (2012) (PubMed).

McCutcheon, Conrad, Carr, Ford, McGehee, Marinelli: "Dopamine neurons in the ventral tegmental area fire faster in adolescent rats than in adults." in: Journal of neurophysiology, Vol. 108, Issue 6, pp. 1620-30, (2012) (PubMed).

Salvatore, Pruett, Dempsey, Fields: "Comprehensive profiling of dopamine regulation in substantia nigra and ventral tegmental area." in: Journal of visualized experiments : JoVE, Issue 66, (2012) (PubMed).

Perez-Costas, Melendez-Ferro, Rice, Conley, Roberts: "Dopamine pathology in schizophrenia: analysis of total and phosphorylated tyrosine hydroxylase in the substantia nigra." in: Frontiers in psychiatry, Vol. 3, pp. 31, (2012) (PubMed).

Nakashima, Mori, Kaneko, Hayashi, Nagatsu, Ota: "Phosphorylation of the N-terminal portion of tyrosine hydroxylase triggers proteasomal digestion of the enzyme." in: Biochemical and biophysical research communications, Vol. 407, Issue 2, pp. 343-7, (2011) (PubMed).

Meyer, Richer, Benkovic, Hayashi, Kansy, Hale, Moy, Kim, OCallaghan, Tsai, Greengard, Nairn, Cowan, Miller, Antich, Bibb: "Striatal dysregulation of Cdk5 alters locomotor responses to cocaine, motor learning, and dendritic morphology." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18561-6, (2008) (PubMed).

Jedynak, Ali, Haycock, Hope: "Acute administration of cocaine regulates the phosphorylation of serine-19, -31 and -40 in tyrosine hydroxylase." in: Journal of neurochemistry, Vol. 82, Issue 2, pp. 382-8, (2002) (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." in: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).

Details zu Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Andere Bezeichnung: Tyrosine Hydroxylase

Hintergrund:

Synonyms: Dystonia 14 antibody, DYT14 antibody, DYT5b antibody, EC 1.14.16.2 antibody, OTTHUMP00000011225 antibody, OTTHUMP00000011226 antibody, ple antibody, Protein Pale antibody, TH antibody, The antibody, TY3H_HUMAN antibody, TYH antibody, Tyrosine 3 hydroxylase antibody, Tyrosine 3 monooxygenase antibody, Tyrosine 3-hydroxylase antibody, Tyrosine 3-monooxygenase antibody, Tyrosine hydroxylase antibody

Description Logic: Our Tyrosine Hydroxylase (Ser40) rabbit polyclonal phosphospecific primary antibody is produced . It detects Tyrosine Hydroxylase (Ser40) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC, ICC.

Gene Name Alternatives: TH

Molekulargewicht: 60 kDa

Gen-ID: 25085

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour