Tryptophan Hydroxylase 2 Antikörper (pSer19)

Produktnummer ABIN361498

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Tryptophan Hydroxylase 2 in WB. Er zeigt eine Reaktivität gegenüber Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für Tryptophan Hydroxylase 2 Antikörper (pSer19) (ABIN361498)

Target: Tryptophan Hydroxylase 2 (TPH2)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tryptophan Hydroxylase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Tryptophan Hydroxylase 2 Antikörper

Bindungsspezifität: pSer19

Spezifität: Specific for the ~55k tryptophan hydroxylase protein phosphorylated at Ser19.

Kreuzreaktivität: Ratte (Rattus)

Homologie: bovine, mouse, zebra fish

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser19 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Referenzen für anti-Tryptophan Hydroxylase 2 Antikörper (ABIN361498)

Matsuo, Izumi, Wakita, Kume, Takada-Takatori, Sawada, Akaike: "Haloperidol, spiperone, pimozide and aripiprazole reduce intracellular dopamine content in PC12 cells and rat mesencephalic cultures: implication of inhibition of vesicular transport." in: European journal of pharmacology, (2010) (PubMed).

Details zu Tryptophan Hydroxylase 2

Target: Tryptophan Hydroxylase 2 (TPH2)

Andere Bezeichnung: TPH2

Hintergrund: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005). Anti-Phospho Ser19 Tryptophan Hydroxylase 2 Western blot of recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of Ca2+/calmodulin dependent kinase II (CaMKII) showing specific immunolabeling of the ~55k tryptophan hydroxylase protein phosphorylated at Ser19.

Molekulargewicht: '55 kDa

Gen-ID: 317675

UniProt: Q8CGU9