RPH3A Antikörper (pSer234)

Produktnummer ABIN361489

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RPH3A in WB und IHC. Er zeigt eine Reaktivität gegenüber Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für RPH3A Antikörper (pSer234) (ABIN361489)

Target: RPH3A (Rabphilin 3A (RPH3A))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RPH3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-RPH3A Antikörper

Bindungsspezifität: pSer234

Spezifität: Specific for the ~82k rabphilin protein phosphorylated at Ser234. The immunolabeling of the rabphilin band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling

Kreuzreaktivität: Ratte (Rattus)

Homologie: mouse

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 IHC: 1:400 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Referenzen für anti-RPH3A Antikörper (ABIN361489)

Stephens, Powell, Taraska, Harris: "Imaging the rapid yet transient accumulation of regulatory lipids, lipid kinases, and protein kinases during membrane fusion, at sites of exocytosis of MMP-9 in MCF-7 cells." in: Lipids in health and disease, Vol. 19, Issue 1, pp. 195, (2020) (PubMed).

Details zu RPH3A

Target: RPH3A (Rabphilin 3A (RPH3A))

Andere Bezeichnung: RPH3A

Hintergrund: Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994,Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease(Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995,Foletti and Scheller, 2001). Anti-Phospho-Ser234 Rabphilin 3A Western blot of rat brain lysate showing specific immunolabeling of the ~82k rabphilin 3A phosphorylated at Ser234.

Molekulargewicht: '82 kDa

Gen-ID: 171039

UniProt: P47709

Pathways: Synaptic Vesicle Exocytosis