MARCKS Antikörper (pSer152, pSer156)

Produktnummer ABIN361478

Produktdetails anti-MARCKS Antikörper

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Bindungsspezifität: pSer152, pSer156

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MARCKS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: Specific for the ~87k MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by -phosphatase

Kreuzreaktivität: Maus, Ratte (Rattus)

Homologie: bovine,chicken, human, Xenopus, zebra fish

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152/156 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Details zu MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Andere Bezeichnung: MARCKS (MARCKS Produkte)

Synonyme: 80K-L antikoerper, MACS antikoerper, PKCSL antikoerper, PRKCSL antikoerper, KINC antikoerper, Macs antikoerper, marcks antikoerper, MGC89127 antikoerper, MARCKS antikoerper, macs antikoerper, wu:fb55c06 antikoerper, wu:fb92d04 antikoerper, wu:fc19b04 antikoerper, wu:fc44a10 antikoerper, zgc:110235 antikoerper, fc34g03 antikoerper, fc38c06 antikoerper, wu:fc34g03 antikoerper, wu:fc38c06 antikoerper, zgc:109978 antikoerper, myristoylated alanine rich protein kinase C substrate antikoerper, myristoylated alanine-rich protein kinase C substrate antikoerper, methyl binding domain106 antikoerper, myristoylated alanine-rich protein kinase C substrate b antikoerper, myristoylated alanine-rich protein kinase C substrate S homeolog antikoerper, myristoylated alanine-rich protein kinase C substrate a antikoerper, MARCKS antikoerper, Marcks antikoerper, marcks antikoerper, mbd106 antikoerper, marcksb antikoerper, marcks.S antikoerper, marcksa antikoerper

Hintergrund: Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al., 1990). The phosphorylation of Ser152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 (Palmer et al., 1996) MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994). Anti-Phospho Ser152/156 MARCKS Western blot of rat brain lysate showing specific immunolabeling of the ~87k MARCKS protein phosphorylated at Serr152,156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the MARCKS Ser152,156 antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Molekulargewicht: '87 kDa

Gen-ID: 3028

UniProt: P30009