GAP43 Antikörper (pSer41)

Produktnummer ABIN361475

Produktdetails anti-GAP43 Antikörper

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Bindungsspezifität: pSer41

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAP43 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: Specific for the ~50k Gap-43 protein phosphorylated at Ser41. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, that may be a Gap-43 aggregate or oligomer. Immunolableing is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is completely eliminated by treatment with (-Ptase.

Kreuzreaktivität: Maus, Ratte (Rattus)

Homologie: bovine, canine, chicken, finch, human, non-human primates, Xenopus, zebra fish

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser41 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Referenzen für anti-GAP43 Antikörper (ABIN361475)

Yi, Kim, Park, Yu, Kim: "The effect of systemic injection of cyclosporin A on the phosphorylation of the PKC substrates MARCKS and GAP43 in the rat hippocampus." in: Neuroscience letters, Vol. 497, Issue 1, pp. 17-21, (2011) (PubMed).

Details zu GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Andere Bezeichnung: GAP43 (GAP43 Produkte)

Hintergrund: Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C (Catalog No. 1609-PKC) modulates the interaction of Gap-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002). Anti-Phospho Ser41 Gap-43 Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap-43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the GAP-43 Ser41 antibody. The immunolabeling of GAP-43 is completely eliminated by treatment with (-Ptase.

Molekulargewicht: '50 kDa

Gen-ID: 29423

UniProt: P07936