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GAP43 Antikörper (pSer41)

Der Kaninchen Polyklonal Anti-GAP43-Antikörper wurde für WB validiert. Er ist geeignet, GAP43 in Proben von Ratte zu detektieren. Es ist 1 Publikation verfügbar.
Produktnummer ABIN361475

Kurzübersicht für GAP43 Antikörper (pSer41) (ABIN361475)

Target

Alle GAP43 Antikörper anzeigen
GAP43 (Growth Associated Protein 43 (GAP43))

Reaktivität

  • 118
  • 113
  • 107
  • 18
  • 9
  • 8
  • 6
  • 5
  • 5
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Ratte

Wirt

  • 109
  • 22
  • 6
  • 2
  • 2
Kaninchen

Klonalität

  • 108
  • 33
Polyklonal

Konjugat

  • 88
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser GAP43 Antikörper ist unkonjugiert

Applikation

  • 90
  • 50
  • 35
  • 28
  • 28
  • 27
  • 27
  • 26
  • 25
  • 25
  • 17
  • 4
Western Blotting (WB)
  • Bindungsspezifität

    • 33
    • 16
    • 10
    • 10
    • 6
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer41

    Spezifität

    Specific for the ~50k Gap-43 protein phosphorylated at Ser41. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, that may be a Gap-43 aggregate or oligomer. Immunolableing is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is completely eliminated by treatment with (-Ptase.

    Kreuzreaktivität

    Maus, Ratte (Rattus)

    Homologie

    bovine, canine, chicken, finch, human, non-human primates, Xenopus, zebra fish

    Aufreinigung

    Antigen Affinity Purified from Pooled Serum

    Immunogen

    Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser41 conjugated to KLH
  • Applikationshinweise

    Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

    Lagerung

    -20 °C
  • Yi, Kim, Park, Yu, Kim: "The effect of systemic injection of cyclosporin A on the phosphorylation of the PKC substrates MARCKS and GAP43 in the rat hippocampus." in: Neuroscience letters, Vol. 497, Issue 1, pp. 17-21, (2011) (PubMed).

  • Target

    GAP43 (Growth Associated Protein 43 (GAP43))

    Andere Bezeichnung

    GAP43

    Hintergrund

    Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C (Catalog No. 1609-PKC) modulates the interaction of Gap-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002). Anti-Phospho Ser41 Gap-43 Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap-43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the GAP-43 Ser41 antibody. The immunolabeling of GAP-43 is completely eliminated by treatment with (-Ptase.

    Molekulargewicht

    '50 kDa

    Gen-ID

    29423

    UniProt

    P07936
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