GABBR2 Antikörper (pSer783)

Produktnummer ABIN361471

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GABBR2 in WB und IF. Er zeigt eine Reaktivität gegenüber Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für GABBR2 Antikörper (pSer783) (ABIN361471)

Target: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GABBR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-GABBR2 Antikörper

Bindungsspezifität: pSer783

Spezifität: Specific for ~102k GABAB R2 phosphorylated at Ser783. Immunolabeling of the GABAB R2 band is completely blocked by (-phosphatase treatment.

Kreuzreaktivität: Maus, Ratte (Rattus)

Homologie: bovine, canine, chicken, human, non-human primates, Xenopus

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser783 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 IF: 1:500 (Kuramoto et al., 2007) Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Referenzen für anti-GABBR2 Antikörper (ABIN361471)

Marron Fernandez de Velasco, Hearing, Xia, Victoria, Luján, Wickman: "Sex differences in GABABR-GIRK signaling in layer 5/6 pyramidal neurons of the mouse prelimbic cortex." in: Neuropharmacology, Vol. 95, pp. 353-60, (2015) (PubMed).

Details zu GABBR2

Target: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Andere Bezeichnung: GABBR2

Hintergrund: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABAB receptors and phosphorylates S783 in the cytoplasmic tail of the R2 subunit and that S783 plays a critical role in enhancing neuronal survival after ischemia as phosphorylation of S783 is evident in many brain regions and is increased dramatically after ischemic injury to the brain (Kuramoto et al., 2007). Anti-Phospho-Ser783 GABAB R2 Western blot of rat synaptic membrane showing specific immunolabeling of the ~102 k GABAB R2 protein phosphorylated at Ser783 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser783 GABAB antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Molekulargewicht: '102 kDa

Gen-ID: 83633

UniProt: O88871

Pathways: cAMP Metabolic Process