Dynamin 1 Antikörper (pSer774)

Produktnummer ABIN361464

Dieses Anti-Dynamin 1-Antikörper ist ein Schaf Polyklonal-Antikörper zur Detektion von Dynamin 1 in WB, IHC und IF. Geeignet für Ratte.

Kurzübersicht für Dynamin 1 Antikörper (pSer774) (ABIN361464)

Target: Dynamin 1 (DNM1)

Reaktivität: Ratte

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Dynamin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-Dynamin 1 Antikörper

Bindungsspezifität: pSer774

Spezifität: Specific for the ~95k dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by (-phosphatase treatment.

Kreuzreaktivität: Ratte (Rattus)

Homologie: bovine, canine, chicken, human, mouse, non-human primates

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 IF (fixed cultured neurons): 1:1000 IHC (fixed cultured neurons): 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Details zu Dynamin 1

Target: Dynamin 1 (DNM1)

Andere Bezeichnung: DNM1

Hintergrund: Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001, Graham et al., 2002, Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.

Molekulargewicht: '95 kDa

Gen-ID: 140694

UniProt: P21575

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling