GABRA1 Antikörper (N-Term)

Produktnummer ABIN361435

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GABRA1 in WB. Er zeigt eine Reaktivität gegenüber Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für GABRA1 Antikörper (N-Term) (ABIN361435)

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GABRA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-GABRA1 Antikörper

Bindungsspezifität: N-Term

Spezifität: Specific for the ~51k a1-subunit of the GABAA receptor in Western blots of rat brain. Labeling is absent in alpha1-subunit knockout animals.

Kreuzreaktivität: Maus, Ratte (Rattus)

Homologie: bovine, canine, human, non-human primate

Aufreinigung: Antigen Affinity Purified

Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Referenzen für anti-GABRA1 Antikörper (ABIN361435)

Agoglia, Zhu, Ying, Sidhu, Natividad, Wolfe, Buczynski, Contet, Parsons, Roberto, Herman: "Corticotropin-Releasing Factor Receptor-1 Neurons in the Lateral Amygdala Display Selective Sensitivity to Acute and Chronic Ethanol Exposure." in: eNeuro, Vol. 7, Issue 2, (2020) (PubMed).

Details zu GABRA1

Target: GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))

Andere Bezeichnung: GABRA1

Hintergrund: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six (s, four (s and four (s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for (- and (-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a (-subunit is required for benzo-diazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different (-subunits of the receptor (McKernan et al., 2000, Mehta and Ticku, 1998, Ogris et al., 2004, Pöltl et al., 2003). Anti-GABAA Receptor, a1-Subunit Western blot of mouse forebrain lysates from Wild Type (Control) and a1-knockout (a1-K/O) animals showing specific immunolabeling of the ~51k a1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from a1-knockout animals.

Molekulargewicht: '51 kDa

Gen-ID: 29705

UniProt: P62813