GLRA1 Antikörper (N-Term)

Produktnummer ABIN361432

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GLRA1 in WB und IHC. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für GLRA1 Antikörper (N-Term) (ABIN361432)

Target: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLRA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GLRA1 Antikörper

Bindungsspezifität: N-Term

Spezifität: Specific for the ~48k a1- and a2-subunits of the glycine receptor in Western blots of rat spinal cord and brain stem and in cell extracts. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen. Does not recognize other glycine receptor subunits.

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 IHC (frozen sections, unpublished observations): 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: lyophilized

Lagerung: -20 °C

Details zu GLRA1

Target: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Andere Bezeichnung: GLRA1

Hintergrund: Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001, Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002). Anti-Glycine Receptor Western blot of rat spinal cord showing specific immunolabeling of the ~48k (1- and (2-subunits of the glycine receptor. The labeling was absent from a rat hippocampal (hipp) lysate as the glycine receptor is not expressed in the hippocampus.

Molekulargewicht: '48 kDa

Gen-ID: 25674

UniProt: P07727

Pathways: Synaptic Membrane