GAPDHS Antikörper

Produktnummer ABIN361377

Dieses Anti-GAPDHS-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von GAPDHS in WB und IF. Geeignet für Schwein.

Kurzübersicht für GAPDHS Antikörper (ABIN361377)

Target: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Reaktivität: Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GAPDHS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 1D4

Produktdetails anti-GAPDHS Antikörper

Spezifität: Specific for the ~38k Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein.

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Homologie: Avian, bovine

Aufreinigung: Total IgM fraction--concentrated culture supernatant

Immunogen: Purified porcine GAPDH

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1,000 IF: 1:100 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: total IgM fraction + 10 mM Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Details zu GAPDHS

Target: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Andere Bezeichnung: GAPDHS

Hintergrund: Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important house keeping functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription (1). GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease (2,3). Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic (4). Purification: Unpurified, concentrated culture supernatant.

Molekulargewicht: '38 kDa

Gen-ID: 26330

UniProt: O14556

Pathways: Regulation of Carbohydrate Metabolic Process