NEFM Antikörper (C-Term)

Produktnummer ABIN361355

Der Huhn Polyklonal Anti-NEFM-Antikörper wurde für WB und IF validiert. Er ist geeignet, NEFM in Proben von Ratte zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für NEFM Antikörper (C-Term) (ABIN361355)

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Reaktivität: Ratte

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser NEFM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-NEFM Antikörper

Bindungsspezifität: C-Term

Spezifität: Specific for the ~145k Neurofilament M protein.

Kreuzreaktivität: Huhn, Rind (Kuh), Human, Maus, Ratte (Rattus)

Aufreinigung: Total IgY fraction

Immunogen: Preparation containing the extreme C-terminus expressed in and purified from E. Coli

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:5,000 IF: 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: total IgY fraction in PBS + 10 mM Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-NEFM Antikörper (ABIN361355)

Glass, Kelm-Nelson, Szot, Lake, Connor, Ciucci: "Functional characterization of extrinsic tongue muscles in the Pink1-/- rat model of Parkinson disease." in: PloS one, Vol. 15, Issue 10, pp. e0240366, (2020) (PubMed).

Details zu NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Andere Bezeichnung: NEFM

Hintergrund: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2). Anti-Neurofilament M Western blot of rat cortex lysate showing specific immunolableing of the ~ 145k NF-M protein.

Molekulargewicht: '145 kDa

Gen-ID: 24588

UniProt: P12839

Pathways: Brown Fat Cell Differentiation