NEFH Antikörper

Produktnummer ABIN361351

Produktdetails anti-NEFH Antikörper

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reaktivität: Rind (Kuh)

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser NEFH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Spezifität: Specific for the ~200k Neurofilament H protein.

Kreuzreaktivität: Huhn, Säugetier

Aufreinigung: Total IgY fraction

Immunogen: Purified bovine NF-H

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:50,000 IF: 1:25,000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: total IgY fraction in PBS + 10 mM Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-NEFH Antikörper (ABIN361351)

Malara, Lutz, Incearap, Bauer, Cursano, Volbracht, Lerner, Pandey, Delling, Ioannidis, Arévalo, von Bernhardi, Schön, Bockmann, Dimou, Boeckers: "SHANK3 deficiency leads to myelin defects in the central and peripheral nervous system." in: Cellular and molecular life sciences : CMLS, Vol. 79, Issue 7, pp. 371, (2022) (PubMed).

Details zu NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Andere Bezeichnung: NEFH (NEFH Produkte)

Hintergrund: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (2) and Alzheimer's disease (3). Anti-Neurofilament H Left: Western blot of rat cortex lysate showing specific immunolableing of the ~200k NF-H protein. Right: Immunofluorescence of rat cortical neurons and glia showing NF-H staining (red).

Molekulargewicht: '200 kDa

Gen-ID: 4744

UniProt: P12036