DLL1 Antikörper (Internal Region)

Produktnummer ABIN361190

Der Ziege Polyklonal Anti-DLL1-Antikörper wurde für WB, ELISA, FACS und IF validiert. Er ist geeignet, DLL1 in Proben von Human und Ratte zu detektieren.

Kurzübersicht für DLL1 Antikörper (Internal Region) (ABIN361190)

Target: DLL1 (delta-Like 1 (DLL1))

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DLL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-DLL1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: DLL1

Sequenz: ATQRHLTVGE EWSQD

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ATQRHLTVGEEWSQD, from the internal region of the protein sequence according to NP_005609.3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 75 kDa band observed in Mouse Heart and Rat Pancreas lysates (calculated MW of 78.5 kDa according to Mouse NP_031891.2 and 77.4 kDa according to Rat NP_114452.1). An additional band of unknown identity was also consistently observed at

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu DLL1

Target: DLL1 (delta-Like 1 (DLL1))

Andere Bezeichnung: DLL1

Hintergrund: DLL1, delta-like 1 (Drosophila), DELTA1, DL1, Delta, OTTHUMP00000017690, delta (Drosophila)-like 1, delta-like 1, delta-like 1 protein

Gen-ID: 28514, 13388, 84010

NCBI Accession: NP_005609

Pathways: Notch Signalweg, Stem Cell Maintenance