PNPLA3 Antikörper (Internal Region)

Produktnummer ABIN361184

Der Ziege Polyklonal Anti-PNPLA3-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, PNPLA3 in Proben von Human und Maus zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für PNPLA3 Antikörper (Internal Region) (ABIN361184)

Target: PNPLA3 (Patatin-Like phospholipase Domain Containing 3 (PNPLA3))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PNPLA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-PNPLA3 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Pnpla3 / Adiponutrin (mouse)

Sequenz: EHDICPKVKS TN

Spezifität: This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence EHDICPKVKSTN, from the internal region of the protein sequence according to NP_473429.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 8 μg/mL.

Western Blot: Approx 45 kDa band observed in Mouse Adrenal Gland lysates, and also in preliminary testing of Mouse and Human Kidney and Rat Adrenal Gland lysate (calculated MW of 42.6Da according to Mouse NP_473429.2). Recommended concentration: 0.01-0.03

Peptide ELISA: antibody detection limit dilution 1:16000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-PNPLA3 Antikörper (ABIN361184)

Yang, Mottillo, Mladenovic-Lucas, Zhou, Granneman: "Dynamic interactions of ABHD5 with PNPLA3 regulate triacylglycerol metabolism in brown adipocytes." in: Nature metabolism, Vol. 1, Issue 5, pp. 560-569, (2019) (PubMed).

Hao, Ito, Huang, Sae-tan, Lambert, Ross: "Shifts in dietary carbohydrate-lipid exposure regulate expression of the non-alcoholic fatty liver disease-associated gene PNPLA3/adiponutrin in mouse liver and HepG2 human liver cells." in: Metabolism: clinical and experimental, Vol. 63, Issue 10, pp. 1352-62, (2014) (PubMed).

Kumari, Schoiswohl, Chitraju, Paar, Cornaciu, Rangrez, Wongsiriroj, Nagy, Ivanova, Scott, Knittelfelder, Rechberger, Birner-Gruenberger, Eder, Brown, Haemmerle, Oberer, Lass, Kershaw, Zimmermann et al.: "Adiponutrin functions as a nutritionally regulated lysophosphatidic acid acyltransferase. ..." in: Cell metabolism, Vol. 15, Issue 5, pp. 691-702, (2012) (PubMed).

Gustavsson, Soga, Wahlström, Vesterlund, Azimi, Norstedt, Tollet-Egnell: "Sex-dependent hepatic transcripts and metabolites in the development of glucose intolerance and insulin resistance in Zucker diabetic fatty rats." in: Journal of molecular endocrinology, Vol. 47, Issue 2, pp. 129-43, (2011) (PubMed).

Details zu PNPLA3

Target: PNPLA3 (Patatin-Like phospholipase Domain Containing 3 (PNPLA3))

Andere Bezeichnung: Pnpla3

Hintergrund: Pnpla3, patatin-like phospholipase domain containing 3, Adpn, adiponutrin

Molekulargewicht: 42.6kDa

Gen-ID: 80339, 116939, 362972

NCBI Accession: NP_473429