IGFBPI Antikörper

Produktnummer ABIN360899

Der Ratte Monoklonal anti-IGFBPI Antikörper wird verwendet zum Nachweis von IGFBPI in Proben von Maus. Er wurde validiert für WB.

Kurzübersicht für IGFBPI Antikörper (ABIN360899)

Target: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser IGFBPI Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 12D24

Produktdetails anti-IGFBPI Antikörper

Spezifität: This antibody detects IGF-BP1. No cross activity to other IGFBPs.

Aufreinigung: Protein A/G affinity chromatography

Immunogen: Recombinant protein of IGF-BP1

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu IGFBPI

Target: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Andere Bezeichnung: IGFBP1

Hintergrund: Insulin like growth factor binding protein 1 (IGFBP1) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The N terminal and C terminal regions are highly homologous among rat, human and bovine sequences. Contained within IGFBP1 and 2 is an integrin receptor recognition sequence (RGD) that is responsible for promoting cell migration by an IGF independent action. IGFBPs hold a central position in IGF ligand receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. IGFBPs will either inhibit or enhance the biological activities of IGF or act in an IGF independent manner. Post translational modification of IGFBPs, including phosphorylation and proteolysis, will modify the affinities of the binding proteins for IGF and may indirectly regulate IGF actions. IGFBP1 is expressed in liver, decidua, and kidneys and is the major IGF binding protein in human amnionic fluid. In hepatocytes, IGFBP1 production is regulated at the transcriptional level due to the effects of insulin and corticosteriods. IGFBP1 is the major determinant of the level of free IGF in serum. The expression of IGFBP1 is inhibited by insulin, IGFI, and IGFII and is stimulated by glucocorticoids, thyroid hormone, and epidermal growth factor (EGF), indicating an endocrine function. IGFBP1 shows inhibitory actions on cell proliferation and differentiation, presumably by interfering with the interactions between IGF and the IGF receptor (IGFR).Synonyms: IBP-1, IBP1, IGF-binding protein 1, IGFBP-1, Insulin-like growth factor-binding protein 1, PP12, Placental protein 12

Gen-ID: 16006

NCBI Accession: NP_032367

UniProt: P47876

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding