ARTN Antikörper

Produktnummer ABIN360873

Dieses Anti-ARTN-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von ARTN in WB und IHC (fro). Geeignet für Maus.

Kurzübersicht für ARTN Antikörper (ABIN360873)

Target: ARTN (Artemin (ARTN))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser ARTN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 6G32

Produktdetails anti-ARTN Antikörper

Spezifität: This antibody detects Mouse Artemin.

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Aufreinigung: Affinity Chromatography on Protein A/G.

Immunogen: Mouse Recombinant Artemin.

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500-1/1000. Immunohistochemistry on Frozen Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 200 μL sterile PBS (final concentration = 500 μg/mL).

Buffer: 0.2 μm filtered PBS solution

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ARTN

Target: ARTN (Artemin (ARTN))

Andere Bezeichnung: Artemin

Hintergrund: Artemin is a member of the Glia Cel-lDerived Neurotrophic factor (GDNF) family ligands, which include GDNF, Persephin, Artemin, and Neurturin. GDNF family ligands are distant members of the Transforming Growth Factor β (TGFβ) superfamily. Similar to other TGFβ family proteins, Artemin is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxyterminal domain. The carboxy terminal domain of Artemin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active Artemin is a disulfide-linked homodimer of the carboxyterminal 113 amino acid residues. Mature mouse Artemin shares 88.5 % amino acid sequence similarity with human Artemin. Mature Artemin also shares approximately 40 % amino acid sequence identity with the other three members of the GDNF family ligands. Bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFRα1GFRα4) and a common signaling component, cRET (receptor tyrosine kinase). Artemin prefers to bind to GFRα3 and activites the GFRα3RET. However, in the presence of RET, it can bind to GFRα1 as well. Artemin has been shown to promote the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs.Synonyms: ARTN, EVN, Enovin, Neublastin

Molekulargewicht: 24 kDa.

Gen-ID: 11876

NCBI Accession: NP_033841

UniProt: Q9Z0L2