NEK1 Antikörper (C-Term)

Produktnummer ABIN360536

Der Kaninchen Polyklonal Anti-NEK1-Antikörper wurde für WB, IHC (p) und EIA validiert. Er ist geeignet, NEK1 in Proben von Human und Hamster zu detektieren.

Kurzübersicht für NEK1 Antikörper (C-Term) (ABIN360536)

Target: NEK1 (NIMA-Related Kinase 1 (NEK1))

Reaktivität: Human, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NEK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-NEK1 Antikörper

Bindungsspezifität: AA 1173-1202, C-Term

Spezifität: This antibody is specific to NEK1.

Aufreinigung: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide between 1173~1202 amino acids from the C-terminal region of Human NEK1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu NEK1

Target: NEK1 (NIMA-Related Kinase 1 (NEK1))

Andere Bezeichnung: NEK1

Hintergrund: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.Synonyms: NimA-related protein kinase 1, NimA-related protein kinase 10, Renal carcinoma antigen NY-REN-55, Serine/threonine-protein kinase Nek1

Gen-ID: 4750

NCBI Accession: NP_001186326

UniProt: Q96PY6