Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the sequence of human HIPK3 within a 1100-1200.
ELISA: 1/1,000. Immunohistochemistry: 1/50 - 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Target
HIPK3
(Homeodomain Interacting Protein Kinase 3 (HIPK3))
HIPK3 negatively regulates apoptosis by promoting FADD phosphorylation. This kinase enhances androgen receptor-mediated transcription, and may act as a transcriptional corepressor for NK homeodomain transcription factors.Synonyms: Androgen receptor-interacting nuclear protein kinase, DYRK6, FIST3, Fas-interacting serine/threonine-protein kinase, Homeodomain-interacting protein kinase 3, Homolog of protein kinase YAK1, PKY