ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. The CKB isoform, present in many tissues but especially brain, is a cytoplasmic enzyme involved in energy homeostasis. CKB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. Creatine kinase B-driven energy transfer in the brain is important for habituation and spatial learning behaviour, mossy fibre field size and determination of seizure susceptibility. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.Synonyms: B-CK, CKB, Creatine kinase B chain, Creatine kinase B-type, Creatine kinase BB