USP25 Antikörper (Ubiquitin Specific Peptidase 25) (N-Term)

Details for Product anti-USP25 Antibody No. ABIN357562
Antigen
Epitop
N-Term
12
8
8
8
4
3
2
1
1
Reaktivität
Human
67
20
13
Wirt
Kaninchen
62
5
Klonalität
Polyklonal
Konjugat
Dieser USP25 Antikörper ist unkonjugiert
5
5
5
4
4
4
Applikation
Enzyme Immunoassay (EIA), Western Blotting (WB)
63
45
26
7
3
1
1
1
Optionen
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human USP25.
Isotyp Ig Fraction
Spezifität This antibody is specific to USP25 (N-term).
Reinigung Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Antigen
Andere Bezeichnung USP25 (USP25 Antibody Abstract)
Hintergrund Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.Synonyms: Deubiquitinating enzyme 25, USP on chromosome 21, USP21, Ubiquitin carboxyl-terminal hydrolase 25, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25
Molekulargewicht 125749 Da
Gen-ID 29761, 5874
UniProt Q9UHP3
Applikations-hinweise ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Bilder des Herstellers
Image no. 1 for anti-Ubiquitin Specific Peptidase 25 (USP25) (N-Term) antibody (ABIN357562) anti-Ubiquitin Specific Peptidase 25 (USP25) (N-Term) antibody